Genotype-phenotype associations in a nonmodel prokaryote

Measuring growth of tranpsoson mutants under a variety of nutrition and stress conditions helped define the biological functions of nonessential genes of Francisella novicida

Coordinated host responses during pyroptosis

Two conserved secretion pathways are shown to be initiated by caspase-1, lysosome exocytosis, and a parallel pathway resulting in cytokine release, and both enhance the antimicrobial nature of pyroptosis.

Evolution of Burkholderia pseudomallei

Whole genome comparisons of isolates from patients with recurring meliodosis infection identify deletions and deleterious mutations associated with increased antibiotic resistance

2013 National Meeting of the RCE Network

April 7-9, 2013
Seattle, WA
The Westin Hotel

News & Events Research Focus About Us

2013 NIAID RCE National Meeting Information

RCE Researchers in the News

Joseph Mougous (Department of Microbiology, University of Washington) is the co-recipient of the Merck Irving S. Sigal Memorial Award given to a young investigator for "excellence in basic research in microbiology and infectious diseases" Read more...

E. P. (Pete) Greenberg (Department of Microbiology, University of Washington) is the recipient of the D.C. White Research and Mentoring Award in recognition of "distinguished accomplishments in interdisciplinary research and mentoring in microbiology" Read more...

Gram-negative pathogens

Many species of Gram-negative bacteria are pathogenic, or cause disease. The agents presently under study at the NWRCE are those that cause melioidosis, plague, and tularemia.

Innate Immune Response

Ongoing NWRCE studies seek to identify genetic factors contributing to variation in human innate immune responses to pathogens, including environmental pathogens and potential bioweapons.

Product Development

A critical approach to battling infectious disease is the devlopment of antibiotics for treatment or prevention.
The Northwest Regional Center of Excellence (NWRCE) is one of eleven Regional Centers of Excellence for Biodefense and Emerging Infectious Diseases Research. The Center conducts basic and applied research on bacterial pathogenesis with the goal of using that information to develop new treatments, vaccines and related technologies.

The NWRCE is a highly integrated center, with research projects at the University of Washington as well as institutions in the metropolitan, state, and regional area. The emphasis of the NWRCE research program takes advantage of the broad range of our investigators’ experience in Gram-negative bacterial pathogenesis.

Highlights from Recent Publications
Functional genetic screen of human diversity reveals that a methionine salvage enzyme regulates inflammatory cell death
Proc Natl Acad Sci U S A. 2012 Jul 25

Dennis Ko

It is shown that common human variation affecting expression of a single gene can alter susceptibility to two distinct cell death programs, and the same allele that promotes cell death is associated with improved survival of individuals with systemic inflammatory response syndrome. Commentary: Balancing resistance and infection tolerance through metabolic means
Genotype-phenotype associations in a nonmodel prokaryote
MBio. 2012 Mar 20

M Enstrom

To help define the biological functions of nonessential genes of Francisella novicida, the growth of arrayed members of a comprehensive transposon mutant library were measured under a variety of nutrition and stress conditions. The greatest surprise of the analysis was the large number of genotype-phenotype relationships that were not predictable from studies of Escherichia coli and other model species.
A Widespread Bacterial Type VI Secretion Effector Superfamily Identified Using a Heuristic Approach
Cell Host Microbe. 2012 May 17

A Russell

A type VI secretion system (T6SS) of Pseudomonas aeruginosa was shown to deliver cell wall-targeting effectors to neighboring cells. Using parameters derived from experimentally validated bacterial T6SS effectors a phylogenetically disperse superfamily of T6SS-associated peptidoglycan-degrading effectors was identified.