Mass Spectrometry Core

Dr. David R. Goodlett

Department of Medicinal Chemistry
University of Washington, Seattle, WA

The Mass Spectrometry and Biological Structure (MSBS) Core provides support for NWRCE investigators to define function of molecules (i.e. proteins and small molecules) through structural analysis and discovery-based profiling experiments. The supported projects primarily seek to discover new proteins (e.g. therapeutic targets) and lipids (e.g. new vaccine adjuvants) involved in virulence or to define new roles for known molecules.

Specifically, the core determines structures of proteins and protein complexes, determines structures of small molecules and screen libraries, and characterizes Global Protein Regulation. To carry out these aims the core utilizes a number of technologies including, but not limited to, the following:

  • High Performance Liquid Chromatography – Electrospray Ionization – Tandem Mass Spectrometry.
  • Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry.
  • Nuclear Magnetic Resonance Spectroscopy.
  • Computational methods to support interpretation of data.