Transgenic Mouse Core

Lynn Hajjar

Department of Immunology
University of Washington, Seattle, WA

Mice – particularly genetically modified mice – are a crucial resource by which to study the pathogenesis of infectious diseases, including those caused by the Gram-negative bacterial pathogens that are the focus of the NWRCE. Mouse models also allow us to evaluate the effects of therapeutic or prophylactic interventions. Moreover, through the creation of ‘humanized mice’ and mice harboring common variations of human genes that may affect their response to such infections or interventions, it is possible to refine these models to better emulate humans.

The overall objective of this core is to generate, breed, genotype and provide genetically modified mice to investigators of the NWRCE. The core provides the following services to investigators of the NWRCE:

  • Advise on appropriate use of mouse models for studies
  • Provide breeding, husbandry, and genotyping of existing mutant mouse lines
  • Generation of new compound genetic mutants through intercrossing and genotyping of existing mutant mouse lines
  • Generate new genetically modified mice
  • Rapidly generate congenic mice from mice on mixed genetic backgrounds using a 'speed congenics' approach